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Analysis of wax ester molecular species by high performance liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry

SYSNO ASEP	0345947
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Analysis of wax ester molecular species by high performance liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry
Author(s)	Vrkoslav, Vladimír (UOCHB-X)_{RID, ORCID} Urbanová, Klára (UOCHB-X)_RID Cvačka, Josef (UOCHB-X)_{RID, ORCID}
Number of authors	3
Source Title	Journal of Chromatography A. - : Elsevier - ISSN 0021-9673 Roč. 1217, č. 25 (2010), s. 4184-4194
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Beeswax ; Branched esters ; Human hair ; Jojoba ; Mass spectrometry
Subject RIV	CC - Organic Chemistry
R&D Projects	GA203/09/0139 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000278779000027
DOI	10.1016/j.chroma.2009.12.048
Annotation	High chromatographic resolution of wax esters (WEs) was achieved by non-aqueous reversed-phase liquid chromatography on a Nova-Pak C18 column by optimizing the acetonitrile/ethyl acetate mobile phase gradient. The retention behaviour of saturated, unsaturated and methyl-branched WEs was studied in this chromatographic system. The analytes were detected by atmospheric pressure chemical ionization mass spectrometry (APCI-MS). WEs provided simple full-scan spectra with abundant protonated molecules and low-intensity fragments. Collision-induced dissociation (CID) promoted identification of the WE molecular species. The responses of WEs were found to be dependent on the number of double bonds and on the alkyl-chain length. The HPLC/APCIMS was applied for the analysis of the WEs isolated from honeycomb beeswax, jojoba oil and human hair. Good agreement between reported results and the literature data was achieved, with several novel polyunsaturated WEs also being found.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2011

Number of the records: 1