Number of the records: 1

First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic

SYSNO ASEP	0345901
Document Type	O - Others
R&D Document Type	Others
Title	First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic
Author(s)	Přibylová, Jaroslava (BC-A)_{RID, ORCID} Špak, Josef (BC-A)_{RID, ORCID} Kubelková, Darina (BC-A) Petrzik, Karel (BC-A)_{RID, ORCID}
Number of authors	4
Source Title	Plant Disease. - : American Phytopathological Society - ISSN 0191-2917 Roč. 94, č. 8 (2010), s. 1071-1071
Number of pages	1 s.
Language	eng - English
Country	US - United States
Keywords	Virus ; small fruits ; pathogen detection
Subject RIV	EE - Microbiology, Virology
R&D Projects	OC09022 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
UT WOS	000280058900045
DOI	10.1094/PDIS-94-8-1071B
Annotation	A collection of highbush blueberry (Vaccinium corymbosum L.) cultivars was surveyed in 2009. A total of 67 plants of 10 cultivars were observed for typical Blueberry red ringspot virus (BRRV) symptoms and were also assayed for BRRV infection using PCR. Extracted DNA from leaf tissue was used in PCR for BRRV detection with primer pair BRRV15/16. No BRRV symptoms were observed on the plants in early spring, yet BRRV was detected in one symptom-free bush of cv. Darrow by PCR. In July, typical symptoms developed on that and another cv. Darrow bush that was also positive by PCR. DNA fragments were amplified from total nucleic acid samples of both infected blueberry bushes using primers BRRV15/16. The amplicons were sequenced and revealed 97.5% nucleotide sequence identity to the BRRV (GenBank accession number AF404509). The 845 nucleotide sequence of the amplicon has been deposited at GenBank under accession number HM107773.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2013

Number of the records: 1