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Survival of Bifidobacteria Administered to Calves
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SYSNO ASEP 0345856 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Survival of Bifidobacteria Administered to Calves Author(s) Vlková, E. (CZ)
Grmanová, M. (CZ)
Killer, Jiří (UZFG-Y) RID, ORCID
Mrázek, Jakub (UZFG-Y) RID, ORCID
Kopečný, Jan (UZFG-Y) RID, ORCID
Bunešová, V. (CZ)
Rada, V. (CZ)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 55, č. 4 (2010), s. 390-392Number of pages 3 s. Language eng - English Country CZ - Czech Republic Keywords bifidobacteria ; gastrointenistinal microflora ; probiotics Subject RIV GM - Food Processing R&D Projects GA523/08/1091 GA ČR - Czech Science Foundation (CSF) GD525/08/H060 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000281277900021 Annotation Twenty-five bifidobacteria were isolated from feces of calves. Isolates were identified, and their functional properties and antimicrobial activity were determined. From 10 strains with suitable properties rifampicin-resistant mutants (RRBs) were prepared and mixture of RRBs was administered to 2-d-old calves. These strains were identified by sequencing as Bifidobacterium animalis ssp. animalis (6 strains), B. thermophilum (2 strains), B. choerinum (1 strain) and B. longum ssp. suis (1 strain). The control group was without probiotic treatment. Survival ability of administered bifidobacteria was monitored in fecal samples by cultivation on modified TPY agar supplemented with mupirocin, acetic acid, and rifampicin. Administered bifidobacteria survived in gastrointestinal tract of calves for at least 60 d. Other bacteria were also determined after cultivation using fluorescence in situ hybridization (FISH). Bifidobacteria and lactobacilli dominated in fecal microflora. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2011
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