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Alternative polymer precipitants for protein crystallization
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SYSNO ASEP 0345206 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Alternative polymer precipitants for protein crystallization Author(s) Skálová, Tereza (UMCH-V) RID
Dušková, Jarmila (UMCH-V) RID
Hašek, Jindřich (UMCH-V) RID
Kolenko, Petr (UMCH-V) RID
Štěpánková, Andrea (UMCH-V)
Dohnálek, Jan (UMCH-V) RIDSource Title Journal of Applied Crystallography. - : Wiley - ISSN 0021-8898
Roč. 43, č. 4 (2010), s. 737-742Number of pages 6 s. Language eng - English Country GB - United Kingdom Keywords polymer precipitants ; protein crystallization ; X-ray crystallography Subject RIV CD - Macromolecular Chemistry R&D Projects IAA500500701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA305/07/1073 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000279429500011 DOI 10.1107/S0021889810014317 Annotation A set of 16 inexpensive and commercially available polymer precipitants were tested for protein crystallization. Eight of them were found suitable: polyethylene glycol dimethyl ether of molecular weight (MW) 500, 1000 and 2000; di[poly(ethylene glycol)] adipate, MW 900; poly(ethylene glycol-ran-propylene glycol), MW 2500 and 12000; poly(acrylic acid) sodium salt, MW 2100; and polyethylene glycol methyl ether methacrylate, MW 1100. Two new crystallization screens, PolyA and PolyB, were formulated using these eight polymers, each containing 96 solutions – four polymers in combination with 24 common salts and buffers, covering pH values from 4.5 to 9.0. The screens were tested on 29 proteins, 21 of which were crystallized. The tests confirmed the applicability of the eight polymers as precipitants for protein crystallization. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2011
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