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Transient expression of human papillomavirus type 16 virus-like particles in tobacco and tomato using a tobacco rattle virus expression vector

SYSNO ASEP	0345055
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Transient expression of human papillomavirus type 16 virus-like particles in tobacco and tomato using a tobacco rattle virus expression vector
Title	Tranzientní exprese pseudovirionů lidského papilomaviru 16 v tabáku a rajčeti pomocí expresního vektoru odvozeného z Rattle viru tabáku
Author(s)	Šmídková, Markéta (UEB-Q) Müller, M. (DE) Thönes, N. (DE) Piuko, K. (DE) Angelisová, Pavla (UMG-J)_RID Velemínský, Jiří (UEB-Q) Angelis, Karel (UEB-Q)_{RID, ORCID}
Source Title	Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134 Roč. 54, č. 3 (2010), s. 451-460
Number of pages	10 s.
Language	eng - English
Country	NL - Netherlands
Keywords	ELISA ; Lycopersicon esculentum ; Nicotiana benthamiana
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA521/01/1418 GA ČR - Czech Science Foundation (CSF)
	GA521/04/0971 GA ČR - Czech Science Foundation (CSF)
	IAA6038201 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	IBS5038304 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000279366200008
DOI	10.1007/s10535-010-0081-4
Annotation	The major capsid protein L1 of human papillomavirus type 16 (HPV16) was transiently expressed in tobacco (Nicotiana benthamiana and Nicotiana tabacum) and tomato (Lycopersicon esculentum) leaves using Agrobacterium tumefaciens. The expression vector pTV00 was derived from tobacco rattle virus (TRV). The highest L1 expression 15 mg g-1 was achieved when the coding sequence of L1 was optimized for expression in humans that caused an increase of the GC content from 38.2 % in wild type HPV16 to 64.1 % in optimized sequence. L1 monomers readily self-assembled into capsomeres and further into virus like particles (VLPs). Immunological characterization and electron microscopy showed that 89 % of L1 retained this structure also in extracts prepared from freeze-dried leaves. Plant expressed L1 was highly immunogenic without any additional adjuvant as vaccinated mice developed strong humoral and cellular immune response, comparable to that elicited by purified VLPs derived from insect cells.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1