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The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors

SYSNO ASEP	0345026
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors
Author(s)	Kubinová, Šárka (UEM-P)_{RID, ORCID} Horák, Daniel (UMCH-V)_{RID, ORCID} Kozubenko, Nataliya (UEM-P)_RID Vaněček, Václav (UEM-P) Proks, Vladimír (UMCH-V)_{RID, ORCID} Price, J. (GB) Cocks, G. (GB) Syková, Eva (UEM-P)_RID
Source Title	Biomaterials. - : Elsevier - ISSN 0142-9612 Roč. 31, č. 23 (2010), s. 5966-5975
Number of pages	10 s.
Language	eng - English
Country	NL - Netherlands
Keywords	cell adhesion ; laminin ; mesenchymal stem cell
Subject RIV	FH - Neurology
R&D Projects	LC554 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	IAA500390902 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50390703 - UEM-P (2007-2013)
	AV0Z40500505 - UMCH-V (2005-2011)
UT WOS	000279719600007
DOI	10.1016/j.biomaterials.2010.04.040
Annotation	Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with laminin-derived Ac-CGGASIKVAVS-OH peptide sequences have been developed to construct scaffolds that promote cell adhesion and neural differentiation. The Ac-CGGASIKVAVS-OH peptide was immobilized to the sulfhydryl groups by 2,2'-dithiodipyridine linking reagent via 2-[4-(2-pyridyldisulfanyl)butanamido]ethyl methacrylate. Superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds significantly increased the number of attached mesenchymal stem cells and their growth area on the hydrogel surface in the absence and in the presence of serum in the culture medium. Additionally, the Ac-CGGASIKVAVS-OH peptide supported the attachment, proliferation, differentiation and process spreading of human fetal neural stem cells during the first two weeks of expansion and contributed to the formation of a high percentage of more mature neural cells after four weeks of expansion.
Workplace	Institute of Experimental Medicine
Contact	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Year of Publishing	2011

Number of the records: 1