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Probing plant membranes with FM dyes: tracking, dragging or blocking?

SYSNO ASEP	0343962
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Probing plant membranes with FM dyes: tracking, dragging or blocking?
Author(s)	Jelínková, Adriana (UEB-Q)_{RID, ORCID} Malínská, Kateřina (UEB-Q)_{RID, ORCID} Simon, Sibu (UEB-Q)_ORCID Kleine-Vehn, J. (DE) Pařezová, Markéta (UEB-Q) Pejchar, Přemysl (UEB-Q)_{RID, ORCID} Kubeš, Martin (UEB-Q)_{RID, ORCID} Martinec, Jan (UEB-Q)_{RID, ORCID} Friml, J. (DE) Zažímalová, Eva (UEB-Q)_{RID, ORCID} Petrášek, Jan (UEB-Q)_{RID, ORCID}
Source Title	Plant Journal. - : Wiley - ISSN 0960-7412 Roč. 61, č. 5 (2010), s. 883-892
Number of pages	10 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	FM 4-64 ; FM 1-43 ; plasma membrane
Subject RIV	ED - Physiology
R&D Projects	KJB600380604 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000274805600014
DOI	10.1111/j.1365-313X.2009.04102.x
Annotation	Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1