Synthetic N-Acetyl-D-glucosamine Based Fully Branched Tetrasaccharide, a Mimetic of the Endogenous Ligand for CD69, Activates CD69+  Killer Lymphocytes upon Dimerization via a Hydrophilic Flexible Linker

Kovalová, Anna; Ledvina, Miroslav; Šaman, David; Zyka, Daniel; Kubíčková, M.; Žídek, L.; Sklenář, V.; Pompach, P.; Kavan, D.; Bílý, J.; Vaněk, O.; Kubínková, Z.; Libigerová, M.; Ivanová, L.; Antolíková, M.; Mrázek, H.; Rozbeský, D.; Hofbauerová, Kateřina; Křen, Vladimír; Bezouška, K.

doi:https://dx.doi.org/10.1021/jm100055b

Number of the records: 1

Synthetic N-Acetyl-D-glucosamine Based Fully Branched Tetrasaccharide, a Mimetic of the Endogenous Ligand for CD69, Activates CD69+ Killer Lymphocytes upon Dimerization via a Hydrophilic Flexible Linker

1.

SYSNO ASEP	0343794
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Synthetic N-Acetyl-D-glucosamine Based Fully Branched Tetrasaccharide, a Mimetic of the Endogenous Ligand for CD69, Activates CD69+ Killer Lymphocytes upon Dimerization via a Hydrophilic Flexible Linker
Author(s)	Kovalová, Anna (UOCHB-X) Ledvina, Miroslav (UOCHB-X)_RID Šaman, David (UOCHB-X)_{RID, ORCID} Zyka, Daniel (UOCHB-X)_RID Kubíčková, M. (CZ) Žídek, L. (CZ) Sklenář, V. (CZ) Pompach, P. (CZ) Kavan, D. (CZ) Bílý, J. (CZ) Vaněk, O. (CZ) Kubínková, Z. (CZ) Libigerová, M. (CZ) Ivanová, L. (CZ) Antolíková, M. (CZ) Mrázek, H. (CZ) Rozbeský, D. (CZ) Hofbauerová, Kateřina (MBU-M)_ORCID Křen, Vladimír (MBU-M)_{RID, ORCID} Bezouška, K. (CZ)
Number of authors	20
Source Title	Journal of Medicinal Chemistry. - : American Chemical Society - ISSN 0022-2623 Roč. 53, č. 10 (2010), s. 4050-4065
Number of pages	16 s.
Language	eng - English
Country	US - United States
Keywords	CD69 ; mimetic ; tetrasaccharide
Subject RIV	CC - Organic Chemistry
R&D Projects	1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA303/09/0477 GA ČR - Czech Science Foundation (CSF)
	GD305/09/H008 GA ČR - Czech Science Foundation (CSF)
	GP203/09/P024 GA ČR - Czech Science Foundation (CSF)
	KAN200520703 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	KAN200100801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000277766900018
DOI	10.1021/jm100055b
Annotation	The systematic structure-activity studies revealed the fully synthetized tetrasaccharide GlcNAc beta1-3(GlcNAc beta1-4)(GlcNAc beta1-6)GlcNAc and its R-benzyl derivative as the best ligands forCD69 with IC50 as high as 10-9 M. Furthermore, this saccharide dimerized through a hydrophilic flexible linker, turned out to be effective in activating CD69+ lymphocytes. It also proved efficient in enhancing natural killing in vitro, decreasing the growth of tumors in vivo, and activating theCD69+ tumor infiltrating lymphocytes examined ex vivo.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2011

Number of the records: 1