Number of the records: 1

Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

SYSNO ASEP	0343446
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches
Author(s)	Dlouhá, Š. (CZ) Thielsch, A. (DE) Kraus, R.H.S. (DE) Seďa, Jaromír (BC-A)_RID Schwenk, K. (DE) Petrusek, A. (CZ)
Source Title	Hydrobiologia. - : Springer - ISSN 0018-8158 Roč. 643, č. 1 (2010), s. 107-122
Number of pages	16 s.
Action	International Symposium on Cladocera /8./
Event date	21.10.2008-25.10.2008
VEvent location	Aguascalientes
Country	MX - Mexico
Event type	WRD
Language	eng - English
Country	NL - Netherlands
Keywords	Daphnia ; interspecific hybridization ; internal transcribed spacer ; allozyme electrophoresis ; microsatellites
Subject RIV	DA - Hydrology ; Limnology
R&D Projects	GA206/04/0190 GA ČR - Czech Science Foundation (CSF)
	IAA600960901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z60170517 - HBU-Z, BC-A (2005-2011)
UT WOS	000276256500014
DOI	10.1007/s10750-010-0128-8
Annotation	Daphnia galeata, D. longispina and D. cucullata are closely related species which often produce interspecific hybrids. Several marker systems are available for taxon determination in this hybridizing complex, but their performance and reliability has not been systematically assessed. We compared results from identifications by three molecular methods. More than 1,200 individuals from 10 localities in the Czech Republic were identified as parental species or hybrids by allozyme electrophoresis and the analysis of the restriction fragment length polymorphism of the internal transcribed spacer (ITS-RFLP); over 440 of them were additionally analyzed and identified by 12 microsatellite loci. Identification by microsatellite markers corresponded well with allozyme analyses. However, consistent discrepancies between ITS-RFLP and other markers were observed in two out of 10 studied localities. These results warn against its use as a sole identification method in field studies.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1