Number of the records: 1

Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94

SYSNO ASEP	0343421
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94
Author(s)	Prudnikova, T. (CZ) Mozga, T. (CZ) Řezáčová, Pavlína (UOCHB-X)_{RID, ORCID} Chaloupková, R. (CZ) Sato, Y. (JP) Nagata, Y. (JP) Brynda, Jiří (UMG-J)_RID Kutý, Michal (UEK-B) Damborský, J. (CZ) Kutá-Smatanová, Ivana (UEK-B)_RID
Number of authors	10
Source Title	Acta Crystallographica Section F-Structural Biology and Crystallization Communications. - : Wiley - ISSN 1744-3091 F65, č. 4 (2009), s. 353-356
Number of pages	4 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	protein crystallization ; X-ray analysis ; dehalogenase
Subject RIV	CC - Organic Chemistry
R&D Projects	LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
	AV0Z60870520 - UEK-B (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000264770000009
DOI	10.1107/S1744309109007039
Annotation	A novel enzyme, DbeA, belonging to the haloalkane dehalogenase family (EC 3.8.1.5) was isolated from Bradyrhizobium elkani USDA94. In order to understand the unique activity and specificity of DbeA, its mutant variant DbeA1, which carries the unique fragment of DbjA, was also constructed. Both wild-type DbeA and DbeA1 were crystallized using the sitting-drop vapour-diffusion method. The crystals of DbeA belonged to the primitive orthorhombic space group P2(1)2(1)2(1), while the crystals of DbeA1 belonged to the monoclinic space group C2. Diffraction data were collected to 2.2 A resolution for both DbeA and DbeA1 crystals.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2011

Number of the records: 1