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Structural organization of WrbA in apo-and holoprotein crystals

SYSNO ASEP	0343396
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Structural organization of WrbA in apo-and holoprotein crystals
Author(s)	Wolfová, J. (CZ) Kutá-Smatanová, Ivana (UEK-B)_RID Brynda, Jiří (UOCHB-X)_{RID, ORCID} Mesters, J. R. (DE) Lapkouski, M. (CZ) Kutý, Michal (UEK-B) Natalello, A. (IT) Chatterjee, N. (US) Chern, S. Y. (US) Ebbel, E. (US) Ricci, A. (US) Grandori, R. (IT) Ettrich, Rüdiger (UEK-B)_{RID, ORCID, SAI} Carey, J. (US)
Number of authors	14
Source Title	Biochimica Et Biophysica Acta-Proteins and Proteomics. - : Elsevier - ISSN 1570-9639 Roč. 1794, č. 9 (2009), s. 1288-1298
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	twisted open-sheet fold ; electrostatic potential surface ; dimerization ; trichloroacetic acid
Subject RIV	CC - Organic Chemistry
R&D Projects	LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
	AV0Z60870520 - UEK-B (2005-2011)
UT WOS	000270100200002
DOI	10.1016/j.bbapap.2009.08.001
Annotation	Crystal structure of /E. coli/ protein WrbA holoprotein to 2.6 and 2.0 Å resolution, and WrbA apoprotein to 1.85 Å, are refined and analysed comparatively through the lens of flavodoxin structures. The results indicate that differences between apo- and holoWrbA crystal structures are manifested on many levels of protein organization as well as in the FMN-binding sites. Structural changes upon cofactor binding are compared with the monomeric flavodoxins. Analysis of the three crystal structures described here, together with flavodoxin structures, rationalizes functional similarities and differences of the WrbAs relative to flavodoxins, leading to a new understanding of the defining features of WrbAs.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2011

Number of the records: 1