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Surface modification of cyclic olefin copolymers for osteochondral defect repair can increase pro-destructive potential of human chondrocytes in vitro
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SYSNO ASEP 0343392 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Surface modification of cyclic olefin copolymers for osteochondral defect repair can increase pro-destructive potential of human chondrocytes in vitro Author(s) Polanská, M. (CZ)
Hulejová, H. (CZ)
Petrtýl, M. (CZ)
Bastl, Zdeněk (UFCH-W) RID, ORCID
Spirovová, Ilona (UFCH-W) RID
Kruliš, Zdeněk (UMCH-V) RID, ORCID
Horák, Zdeněk (UMCH-V)
Veigl, D. (CZ)
Šenolt, L. (CZ)Source Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 59, č. 2 (2010), s. 247-253Number of pages 7 s. Language eng - English Country CZ - Czech Republic Keywords osteochondral defects ; cycloolefin copolymer ; chondrocytes ; biocompatibility Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GA106/06/0761 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40400503 - UFCH-W (2005-2011) AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000278016200012 Annotation Materials on the basis of cycloolefin copolymers (COC) are suitable for subchondral defect repairs. The objective of this study was to evaluate the influence of surface modification of COC and COC/LLDPE blends on the viability and gene expression of chondrocytes. Human chondrocytes were incubated on the surface of the studied materials. Half of the materials were plasmatically modified with a subsequent type II collagen application. The gene expression of matrix metalloproteinases (MMP-1,-3,-13), pro-inflammatory cytokines (IL-1, TNF-alpha) and apoptotic molecules (BAX, Bcl-2) was evaluated using quantitative Taq-Man PCR after 48 h incubation. Chondrocyte viability was evaluated by the MTT test after 2, 4 and 8 days of incubation. The synthesis of MMPs was measured by ELISA assay in cell culture medium after 48 h of incubation. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2011
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