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Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope
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SYSNO ASEP 0343182 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope Author(s) Reshak, Ali H (UEK-B)
Sarafis, V. (AU)
Heintzmann, R. (DE)Number of authors 3 Source Title Micron. - : Elsevier - ISSN 0968-4328
Roč. 40, č. 3 (2009), s. 378-385Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords 2PLSM ; collagen ; generatin ; scattering ; membrane ; fibrils Subject RIV BO - Biophysics CEZ AV0Z60870520 - UEK-B (2005-2011) UT WOS 000263780400013 DOI 10.1016/j.micron.2008.09.007 Annotation Background: Second harmonic information reveals information about the structure of spatially oriented structures with an asymmetry. We study the second harmonic images of the grana and intergranal parts of chloroplasts in the leaves of the shade moss Plagiomnium affine. Results: A two-photon microscope with blocking filters to suppress fluorescence generated both forward and backward second harmonic illumination to be captured. In the starch free chloroplasts of the single cell layered laminae strong second harmonic generation (SHG) from the granal regions was emitted. Upon illumination the chloroplasts changed their orientation affecting the SHG signal. Chloroplast is reoriented upon illumination. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2011
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