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Lipopolythiourea/DNA interaction: A biophysical study

SYSNO ASEP	0342982
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Lipopolythiourea/DNA interaction: A biophysical study
Author(s)	Kral, Teresa (UFCH-W)_{RID, ORCID} Leblond, J. (FR) Hof, Martin (UFCH-W)_{RID, ORCID} Scherman, D. (FR) Hersovici, J. (FR) Mignet, N. (FR)
Source Title	Biophysical Chemistry. - : Elsevier - ISSN 0301-4622 Roč. 148, 1-3 (2010), s. 68-73
Number of pages	6 s.
Language	eng - English
Country	NL - Netherlands
Keywords	lipopolythiourea ; FCS ; plasmid DNA compaction ; 31P NMR
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	IAA400400621 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
UT WOS	000277743900009
DOI	10.1016/j.bpc.2010.02.011
Annotation	Lipopolythioureas (LPT) are original non cationic systems representing an alternative to cationic lipids. Their high transfection efficiency prompted us to investigate further their biophysical properties, and in particular how thiourea lipids interact with DNA. The interaction of lipopolythiourea with DNA was investigated by fluorescence correlation microscopy (FCS). Influence of the lipid length and nature of the thiourea head on the thiourea/DNA interaction were studied. FCS revealed a strong interaction between lipopolythiourea and DNA, occurring at 1 equivalent of a thiourea lipid by a DNA phosphate group, and leading to a condensed plasmid state. From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2011

Number of the records: 1