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Activation of protective cell-mediated immune response in gastric mucosa during Cryptosporidium muris infection and re-infection in immunocompetent mice

SYSNO ASEP	0342901
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Activation of protective cell-mediated immune response in gastric mucosa during Cryptosporidium muris infection and re-infection in immunocompetent mice
Author(s)	Jalovecká, M. (CZ) Sak, Bohumil (BC-A)_{RID, ORCID} Kváč, Martin (BC-A)_{RID, RID, ORCID} Květoňová, Dana (BC-A)_SAI Kučerová, Z. (US) Salát, Jiří (BC-A)_{RID, ORCID}
Source Title	Parasitology Research. - : Springer - ISSN 0932-0113 Roč. 106, č. 5 (2010), s. 1159-1166
Number of pages	8 s.
Language	eng - English
Country	DE - Germany
Keywords	Cryptosporidium muris ; T-lymphocyte migration ; gastric mucosa
Subject RIV	GJ - Animal Vermins ; Diseases, Veterinary Medicine
R&D Projects	KJB500960701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000276234300021
DOI	10.1007/s00436-010-1785-2
Annotation	The immune response in the stomach mucosa during the primary infection and re-infection with Cryptosporidium muris (TS03 and CB03) in immunocompetent BALB/c mice was characterized using flow cytometry analysis and measurement of IFN-gamma and IL10 by enzyme-linked immunosorbent assays (ELISA). Significantly, elevated migration of T lymphocytes (more than 1,000-fold), especially CD8+ T lymphocytes, to the stomach mucosa occurred during primary infection and persisted for more than 2 months after its resolution. The ex vivo cultures of splenocytes revealed very low levels of IFN-gamma production during the course of the primary infection (0.5 ng/ml), whereas in the following re-exposure to the parasites, the concentration of IFN-gamma rapidly increased 22-fold. These results imply that the CD8+ T lymphocytes are involved in the immune response to gastric cryptosporidiosis and could play an important role in the elimination of C. muris infection in mice.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1