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Filamoeba sp. Isolated from Hot-water Piping System, a Host of Legionella Closely Related to Legionella micdadei
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SYSNO ASEP 0342895 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Filamoeba sp. Isolated from Hot-water Piping System, a Host of Legionella Closely Related to Legionella micdadei Author(s) Dyková, Iva (BC-A) RID
Hrubá, L. (CZ)
Kostka, Martin (PAU-O)
Pecková, Hana (BC-A) RID, ORCIDSource Title Acta Protozoologica. - : Jagellonian University - ISSN 0065-1583
Roč. 48, č. 4 (2009), s. 321-327Number of pages 7 s. Language eng - English Country PL - Poland Keywords Filamoeba sp. ; intracellular ; Legionella sp. Subject RIV GJ - Animal Vermins ; Diseases, Veterinary Medicine R&D Projects GA524/09/0137 GA ČR - Czech Science Foundation (CSF) LC522 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000274737300002 Annotation An amoeba was isolated from 56 ºC hot water, sampled in a piping system that was monitored for the presence of legionellae. The amoeba strain JIH56 was derived from the primary isolate by subculturing at 38ºC. It was assigned to the genus Filamoeba Page, 1967 using light microscopical, ultrastructural and molecular criteria. In the cytoplasm of Filamoeba trophozoites were found Legionella-like bacteria. These were determined using Legionella-specific PCR assay followed by sequencing. Phylogenetic analysis and a similarity search revealed a close relationship of the newly determined legionella sequence with sequences of Legionella micdadei (syn. Tatlockia micdadei), the Pittsburgh pneumonia agent. Phylogenetic analysis of the Filamoeba sequences available to date suggests that the ATCC 50430 strain was misidentified. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
Number of the records: 1