Number of the records: 1

Intra-individual internal transcribed spacer 1 (ITS1) and ITS2 ribosomal sequence variation linked with multiple rDNA loci: A case of triploid Atractolytocestus huronensis, the monozoic cestode of common carp

SYSNO ASEP	0342847
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Intra-individual internal transcribed spacer 1 (ITS1) and ITS2 ribosomal sequence variation linked with multiple rDNA loci: A case of triploid Atractolytocestus huronensis, the monozoic cestode of common carp
Author(s)	Kraľová-Hromadová, I. (SK) Štefka, Jan (BC-A)_{RID, ORCID} Špakulová, M. (SK) Orosová, Martina (BC-A) Bombarová, M. (SK) Hanzelová, V. (SK) Bazsalovicsová, E. (SK) Scholz, Tomáš (BC-A)_{RID, ORCID}
Source Title	International Journal for Parasitology. - : Elsevier - ISSN 0020-7519 Roč. 40, č. 2 (2010), s. 175-181
Number of pages	7 s.
Language	eng - English
Country	AU - Australia
Keywords	Fish parasites ; Caryophyllidea ; Ribosomal divergence ; Karyotype ; FISH
Subject RIV	GJ - Animal Vermins ; Diseases, Veterinary Medicine
R&D Projects	LC522 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA524/08/0885 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000274447900005
DOI	10.1016/j.ijpara.2009.07.002
Annotation	Complete sequences of the ribosomal internal transcribed spacers (ITS1 and ITS2) and karyological characters of the monozoic (unsegmented) tapeworm Atractolytocestus huronensis Anthony, 1958 (Cestoda: Caryophyllidea) from Slovakia were analysed, revealing considerable intra-genomic variability and triploidy in all analysed specimens. In individual tapeworms, two to four ITS1 and three to four ITS2 sequence types were found. Divergent intra-genomic ITS copies were mostly induced by nucleotide substitutions and different numbers of short repetitive motifs within the sequence. In addition, triploidy was found to be a common feature of A. huronensis. The karyotype of Slovakian A. huronensis possesses three sets of chromosomes (3n = 24, n = 4m + 3st + 1 minute chromosome). Fluorescent in situ hybridisation (FISH) with a ssrDNA probe revealed two distinct rDNA clusters for each homologue of the triplet number 2.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1