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Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes
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SYSNO ASEP 0342763 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes Author(s) Macháň, Radek (UFCH-W)
Hof, Martin (UFCH-W) RID, ORCIDSource Title International Journal of Molecular Sciences. - : MDPI
Roč. 11, č. 2 (2010), s. 427-457Number of pages 31 s. Language eng - English Country US - United States Keywords lateral diffusion ; fluorescence fluctuation spectroscopy ; confocal microscopy Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GA203/08/0114 GA ČR - Czech Science Foundation (CSF) GEMEM/09/E006 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000274973500003 DOI 10.3390/ijms11020427 Annotation Fluorescence correlation spectroscopy (FCS) is a single molecule technique used mainly for determination of mobility and local concentration of molecules. This review describes the specific problems of FCS in planar systems and reviews the state of the art experimental approaches such as 2-focus, Z-scan or scanning FCS, which overcome most of the artefacts and limitations of standard FCS. We focus on diffusion measurements of lipids and proteins in planar lipid membranes and review the contributions of FCS to elucidating membrane dynamics and the factors influencing it, such as membrane composition, ionic strength, presence of membrane proteins or frictional coupling with solid support. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2011
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