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Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation

SYSNO ASEP	0342588
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation
Author(s)	Bříza, Jindřich (BC-A)_RID Růžičková, N. (CZ) Niedermeierová, Hana (BC-A) Dusbábková, Jana (BC-A) Vlasák, Josef (BC-A)_{RID, ORCID}
Number of authors	5
Source Title	Acta Biochimica Polonica - ISSN 0001-527X Roč. 57, č. 1 (2010), s. 63-68
Number of pages	6 s.
Language	eng - English
Country	PL - Poland
Keywords	pmi ; npt II ; Lactuca sativa
Subject RIV	EB - Genetics ; Molecular Biology
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
UT WOS	000276109500008
Annotation	A positive selection system using phosphomannose isomerase was employed for Agrobacterium tumefaciens mediated transformation of lettuce (Lactuca sativa L. var. ‘Achát’). It was shown that the mannose-based selection system works very well with the lettuce genotype used, reaching up to 25 % transformation efficiency on the medium with 20 g/L mannose and 20 g/L sucrose.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1