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Protein alterations induced by long-term agonist treatment of HEK293 cells expressing thyrotropin-releasing hormone receptor and G11alpha protein
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SYSNO ASEP 0342117 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Protein alterations induced by long-term agonist treatment of HEK293 cells expressing thyrotropin-releasing hormone receptor and G11alpha protein Author(s) Drastichová, Z. (CZ)
Bouřová, Lenka (FGU-C)
Hejnová, L. (CZ)
Jedelský, P. (CZ)
Svoboda, Petr (FGU-C) RID, ORCID
Novotný, J. (CZ)Source Title Journal of Cellular Biochemistry. - : Wiley - ISSN 0730-2312
Roč. 109, č. 1 (2010), s. 255-264Number of pages 10 s. Language eng - English Country US - United States Keywords Thyrotropin-releasing hormone ; Gq/11 protein ; proteomics Subject RIV ED - Physiology R&D Projects LC554 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA309/06/0121 GA ČR - Czech Science Foundation (CSF) GD305/08/H037 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000273339200027 DOI 10.1002/jcb.22409 Annotation This study aimed to determine protein alterations induced by thyrotropin-releasing hormone in plasma membrane-enriched fraction of HEK293, which was prepared by Percoll gradient centrifugation. The high enrichment in plasma membrane proteins of this preparation was confirmed by a multifold increase in the number of TRH receptors and agonist stimulated G-protein activity, compared to postnuclear supernatant. Using 2D-electrophoresis we were able to determine 61 altered proteins in this fraction isolated from cells treated with TRH (1x10-5 M, 16 h), some of which belong to antiapoptotic proteins and their increased amount after TRH treatment were in line with the presumed antiapoptotic effect of TRH Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2011
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