Number of the records: 1

Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'

SYSNO ASEP	0342115
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'
Author(s)	Fránová, Jana (BC-A)_{RID, ORCID} Petrzik, Karel (BC-A)_{RID, ORCID}
Number of authors	2
Source Title	Journal of Phytopathology - Phytopathologische Zeitschrift. - : Wiley - ISSN 0931-1785 Roč. 158, č. 4 (2010), s. 317-320
Number of pages	4 s.
Language	eng - English
Country	DE - Germany
Keywords	16S Ribosomal-RNA ; mycoplasmalike organisms ; gene-sequences
Subject RIV	EE - Microbiology, Virology
R&D Projects	1QS500510558 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
UT WOS	000275396600016
DOI	10.1111/j.1439-0434.2009.01615.x
Annotation	Asparagus officinalis plants with severe fasciation of some spears were observed in southern Bohemia between 1998 and 2007. Nucleic acids extracted from these and asymptomatic plants were assayed with nested polymerase chain reaction (PCR) using the phytoplasma-specific universal ribosomal primers P1/P7 and R16F2n/R2. The restriction profiles obtained from digestion of the PCR products with five endonucleases (AluI, HhaI, KpnI, MseI and RsaI) were identical in all phytoplasmas infecting asparagus in the Czech Republic and indistinguishable from those of phytoplasmas in the aster yellows group (subgroup 16SrI-B). Sequence analysis of 1754 bp of the ribosomal operon indicated that the closest related phytoplasmas were those associated with epilobium phyllody and onion yellows. This is the first report of the natural occurrence of 'Candidatus Phytoplasma asteris' in A. officinalis.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1