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Detection of condensin I and II in maturing pig oocytes

SYSNO ASEP	0341856
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Detection of condensin I and II in maturing pig oocytes
Author(s)	Lišková, Lucie (UZFG-Y) Šušor, Andrej (UZFG-Y)_{RID, ORCID} Pivoňková, Kateřina (UZFG-Y) Šašková, Adéla (UZFG-Y) Karabínová, Pavla (UZFG-Y)_RID Kubelka, Michal (UZFG-Y)_{RID, ORCID}
Source Title	Reproduction Fertility and Development. - : CSIRO Publishing - ISSN 1031-3613 Roč. 22, č. 4 (2010), s. 644-652
Number of pages	9 s.
Language	eng - English
Country	AU - Australia
Keywords	Chromosome condensation ; Female meiosis ; Phosphorylation
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA204/06/1297 GA ČR - Czech Science Foundation (CSF)
	GD204/09/H084 GA ČR - Czech Science Foundation (CSF)
	GP524/09/P435 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000276106000007
DOI	10.1071/RD09068
Annotation	The multiprotein complexes known as condensins (I and II) are major players in chromosome dynamics in mitotic and meiotic cells. Here, we report for the first time the detection of different condensin subunits from both complexes in mammalian oocytes. Using immunoblotting analysis we examined expression levels of condensin subunits during meiotic maturation of porcine oocytes. The expression of the core subunit structural maintenance of chromosomes 2 (SMC2), identical in both condensin complexes, did not change significantly during maturation. Similarly, there was no significant change in the expression of the chromosome associated protein (CAP)-H and CAP-H2 subunits, components of condensin I and II, respectively. Conversely, the expression profiles of CAP-G, CAP-D2 (condensin I) and CAP-D3 (condensin II) were more interesting. At least two isoforms of the CAP-D2 subunit were detected, along with three isoforms of the CAP-D3 and CAP-G subunits.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2011

Number of the records: 1