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The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod

SYSNO ASEP	0341849
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod
Author(s)	Čebašek, V. (SI) Eržen, I. (SI) Vyhnal, Aleš (FGU-C) Janáček, Jiří (FGU-C)_{RID, ORCID} Ribarič, S. (SI) Kubínová, Lucie (FGU-C)_{RID, ORCID}
Source Title	Microvascular Research. - : Elsevier - ISSN 0026-2862 Roč. 79, č. 1 (2010), s. 40-46
Number of pages	7 s.
Language	eng - English
Country	US - United States
Keywords	confocal microscopy ; capillaries ; length measurement
Subject RIV	EA - Cell Biology
R&D Projects	MEB090910 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GD204/09/H084 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000274055200006
DOI	10.1016/j.mvr.2009.11.005
Annotation	We compared two methods of evaluation of capillary supply of individual skeletal muscle fibers, the traditional one using 2D images of thin transverse sections by the number of capillary profiles around a fiber (CAF) and the 3D approach where the mean length of capillaries around individual muscle fibers per fiber length (Lcap/Lfib) is measured from 3D images acquired by confocal microscopy. In models we showed that Lcap/Lfib was sensitive to different arrangements of capillaries, while CAF underestimated capillarization since it could not detect the increased length of capillary bed. In true muscle samples, we detected statistically significant differences in the capillary supply of control and denervated rat soleus muscles by both 2D and 3D methods. Lcap/Lfib was larger than CAF in control muscles reflecting their more complicated capillary bed
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2011

Number of the records: 1