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Modulation of AMPA excitatory postsynaptic currents in the spinal cord dorsal horn neurons by insulin

SYSNO ASEP	0341812
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Modulation of AMPA excitatory postsynaptic currents in the spinal cord dorsal horn neurons by insulin
Author(s)	Špicarová, Diana (FGU-C)_{RID, ORCID} Paleček, Jiří (FGU-C)_{RID, ORCID}
Source Title	Neuroscience. - : Elsevier - ISSN 0306-4522 Roč. 166, č. 1 (2010), s. 305-311
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	pain ; synaptic modulation ; insulin
Subject RIV	FH - Neurology
R&D Projects	GA305/06/1115 GA ČR - Czech Science Foundation (CSF)
	GA305/09/1228 GA ČR - Czech Science Foundation (CSF)
	LC554 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000274507500031
DOI	10.1016/j.neuroscience.2009.12.007
Annotation	Glutamate AMPA receptors are critical for sensory transmission at the spinal cord dorsal horn (DH). Plasma membrane AMPA receptor endocytosis that can be induced by insulin may underlie long term modulation of synaptic transmission. In this work the effect of insulin application on fast excitatory postsynaptic currents (EPSCs) mediated by AMPA receptors evoked in DH neurons was evaluated. Acute spinal cord slices from 6 to 10 day old mice were used to record EPSCs evoked in visually identified superficial DH neurons by dorsal root primary afferent stimulation. AMPA EPSCs could be evoked in all of the tested neurons. In 75% of the neurons the size of the AMPA EPSCs was reduced to 62.1% and to 68.9% of the control values when 0.5 or 10 _M insulin was applied.Our results suggest a possible role of the insulin pathway in modulation of sensory and nociceptive synaptic transmission in the spinal cord.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2011

Number of the records: 1