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Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes

SYSNO ASEP	0341720
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes
Author(s)	Filippov, Sergey K. (UMCH-V)_{RID, ORCID, SAI} Koňák, Čestmír (UMCH-V)_RID Kopečková, P. (US) Starovoytova, Larisa (UMCH-V)_RID Špírková, Milena (UMCH-V)_{RID, ORCID} Štěpánek, Petr (UMCH-V)_{RID, ORCID}
Source Title	Langmuir. - : American Chemical Society - ISSN 0743-7463 Roč. 26, č. 7 (2010), s. 4999-5006
Number of pages	8 s.
Language	eng - English
Country	US - United States
Keywords	DNA ; polyplex ; cholesterol
Subject RIV	BO - Biophysics
R&D Projects	GA202/09/2078 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40500505 - UMCH-V (2005-2011)
UT WOS	000275995100068
DOI	10.1021/la9036716
Annotation	Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. DLS, SLS, electrophoretic light scattering, SAXS, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30-60 nm. The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2011

Number of the records: 1