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Photobioreactor for cultivation and real-time, in-situ measurement of O-2 and CO2 exchange rates, growth dynamics, and of chlorophyll fluorescence emission of photoautotrophic microorganisms
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SYSNO ASEP 0341130 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Photobioreactor for cultivation and real-time, in-situ measurement of O-2 and CO2 exchange rates, growth dynamics, and of chlorophyll fluorescence emission of photoautotrophic microorganisms Author(s) Červený, Jan (UEK-B) RID, ORCID, SAI
Šetlík, Ivan (MBU-M)Number of authors 4 Source Title Engineering in Life Sciences. - : Wiley - ISSN 1618-0240
Roč. 3, č. 9 (2009), s. 247-253Number of pages 7 s. Language eng - English Country DE - Germany Keywords PHOTOSYNTHESIS ; Bioreactor ; Biotechnology Subject RIV CE - Biochemistry R&D Projects GA206/05/0894 GA ČR - Czech Science Foundation (CSF) GA206/09/1284 GA ČR - Czech Science Foundation (CSF) GA206/08/1683 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z60870520 - UEK-B (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000268818500013 Annotation A detailed knowledge about the dynamics of phytoplanktonic photosynthesis and respiration is crucial for the determination of primary productivity in oceans as well as for biotechnology. The dynamics are best studied in photobioreactors that are able to simulate natural conditions. We demonstrate performance of a newly designed laboratory photobioreactor that meets these needs, with a study of green alga Scenedesmusquadricauda. The instrument monitored the culture growth by measuring the optical densities at 735 and 680 nm and by measuring several fluorescence parameters. These widely used but rather indirect estimates were confronted with direct measurements of O2 quantum yields. The CO2 fluxes in and out of the culture media as well as the dissolved CO2 in algal suspension were also recorded. The experiments demonstrated potential of the photobioreactor to reveal minute modulations in gas exchange rates as well as to yield data for planning of large scale photobioreactors. A detailed knowledge about the dynamics of phytoplanktonic photosynthesis and respiration is crucial for the determination of primary productivity in oceans as well as for biotechnology. The dynamics are best studied in photobioreactors that are able to simulate natural conditions. We demonstrate performance of a newly designed laboratory photobioreactor that meets these needs, with a study of green alga Scenedesmusquadricauda. The instrument monitored the culture growth by measuring the optical densities at 735 and 680 nm and by measuring several fluorescence parameters. These widely used but rather indirect estimates were confronted with direct measurements of O2 quantum yields. The CO2 fluxes in and out of the culture media as well as the dissolved CO2 in algal suspension were also recorded. The experiments demonstrated potential of the photobioreactor to reveal minute modulations in gas exchange rates as well as to yield data for planning of large scale photobioreactors. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2010
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