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Mutasynthesis of lincomycin derivatives with activity against drug-resistant staphylococci

SYSNO ASEP	0340856
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Mutasynthesis of lincomycin derivatives with activity against drug-resistant staphylococci
Author(s)	Ulanová, Dana (MBU-M) Novotná, Jitka (MBU-M) Smutná, Yvona (MBU-M) Kameník, Zdeněk (MBU-M)_{RID, ORCID} Gažák, Radek (MBU-M)_{RID, ORCID} Šulc, Miroslav (MBU-M)_{RID, ORCID} Sedmera, Petr (MBU-M) Kadlčík, Stanislav (MBU-M)_{RID, ORCID} Plháčková, Kamila (MBU-M)_RID Janata, Jiří (MBU-M)_{RID, ORCID}
Source Title	Antimicrobial Agents and Chemotherapy. - : American Society for Microbiology - ISSN 0066-4804 Roč. 54, č. 2 (2010), s. 927-930
Number of pages	4 s.
Language	eng - English
Country	US - United States
Keywords	COAGULASE-NEGATIVE STAPHYLOCOCCI ; ERYTHROMYCIN RESISTANCE ; SUBSTRATE-SPECIFICITY
Subject RIV	EE - Microbiology, Virology
R&D Projects	2B08064 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	IAA500200810 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000273860600049
DOI	10.1128/AAC.00918-09
Annotation	The lincomycin biosynthetic gene lmbX was deleted in Streptomyces lincolnensis ATCC 25466 that led to abolition of lincomycin production. Complementation experiments proved the blockage in biosynthesis of lincomycin precursor 4-propyl-L proline. Feeding this mutant strain with precursor derivatives resulted in production of 4-butyl-4-depropyllincomycin and 4-pentyl-4-depropyllincomycin in high titers and without lincomycin contamination. Moreover, 4-pentyl-4-depropyllincomycin was found to be more active than lincomycin against clinical Staphylococcus isolates with genes determining low-level lincosamide resistance
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2010

Number of the records: 1