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Condensation reactions catalyzed by α-N-acetylgalactosaminidase from Aspergillus niger yielding a-N-acetylgalactosaminides
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SYSNO ASEP 0340836 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Condensation reactions catalyzed by α-N-acetylgalactosaminidase from Aspergillus niger yielding a-N-acetylgalactosaminides Author(s) Weignerová, Lenka (MBU-M) RID
Pelantová, Helena (MBU-M) ORCID, RID
Manglová, Dana (MBU-M)
Michálková, Klára (MBU-M)
Křen, Vladimír (MBU-M) RID, ORCIDSource Title Biocatalysis and Biotransformation - ISSN 1024-2422
Roč. 28, č. 2 (2010), s. 150-155Number of pages 6 s. Language eng - English Country GB - United Kingdom Keywords alpha-N-Acetylgalactosaminidase ; amino acid glycosylation ; enzymatic glycosylation Subject RIV EE - Microbiology, Virology R&D Projects OC 136 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) UT WOS 000274855300008 DOI 10.3109/10242421003587227 Annotation Extracellular a-N-acetylgalactosaminidase from Aspergillus niger catalyzed glycosylation yielding a series of 2-acetamido-2-deoxy-a-D-galactobiosides using 2-acetamido-2-deoxy-D-galactopyranose as a glycosyl donor. The isomers a-D-GalpNAc-D-GalpNAc, a-D-GalpNAc-D-GalpNAc and a-D-GalpNAc-D-GalfNAc were isolated and spectrally characterized. The purified enzyme was further used for the glycosylation of free amino acids (serine and threonine) and their N-(tert-butoxycarbonyl)-protected analogs to synthesize the Tn antigen (GalpNAc-a-O-Ser/Thr) and its N-(tertbutoxycarbonyl)-protected derivatives Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2010
Number of the records: 1