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Bid integrates intrinsic and extrinsic signaling in apoptosis induced by alpha-tocopheryl succinate in human gastric carcinoma cells

SYSNO ASEP	0340714
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Bid integrates intrinsic and extrinsic signaling in apoptosis induced by alpha-tocopheryl succinate in human gastric carcinoma cells
Author(s)	Zhao, Y. (CN) Li, R. (CN) Xia, W. (CN) Neužil, Jiří (BTO-N)_RID Lu, Y. (CN) Zhang, H. (CN) Zhao, X. (CN) Zhang, X. (CN) Sun, C. (CN) Wu, K. (CN)
Source Title	Cancer letters. - : Elsevier - ISSN 0304-3835 Roč. 288, č. 1 (2010), s. 42-49
Number of pages	8 s.
Language	eng - English
Country	IE - Ireland
Keywords	Alpha-tocopheryl succinate ; signaling ; apoptosis
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA204/08/0811 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50520701 - BTO-N (2007-2013)
UT WOS	000274991700006
DOI	10.1016/j.canlet.2009.06.021
Annotation	The underlying mechanisms of alpha-tocopheryl succinate (alpha-TOS)-mediated apoptosis are not understood in detail, although the redox-silent vitamin E analog is a potent apoptogen and anti-cancer agent. The objective of the present study was to investigate whether apoptosis triggered by alpha-TOS in gastric carcinomas cells involves both mitochondria- and death receptor-dependent pathways. alpha-TOS induced apoptosis and mitochondrial permeability transition in a concentration- and time-dependent manner. As a consequence, cytochrome c and the apoptosis-inducing factor were released and caspases were activated. Bax was translocated from the cytosol to mitochondria and Bid was cleaved into its truncated form, tBid. Knocking down Bid by RNAi and Fas antisense oligodeoxynucleotides resulted in a decreased release and cleavage. The results imply that Bid may serve as a critical integrating factor of the death receptor and mitochondrial pathway in alpha-TOS-mediated apoptosis.
Workplace	Institute of Biotechnology
Contact	Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
Year of Publishing	2010

Number of the records: 1