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Robust Up-Regulation of Nuclear Red Fluorescent-Tagged Fos Marks Neuronal Activation in Green Fluorescent Vasopressin Neurons after Osmotic Stimulation in a Double-Transgenic Rat
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SYSNO ASEP 0340575 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Robust Up-Regulation of Nuclear Red Fluorescent-Tagged Fos Marks Neuronal Activation in Green Fluorescent Vasopressin Neurons after Osmotic Stimulation in a Double-Transgenic Rat Author(s) Fujihara, H. (JP)
Ueta, Y. (JP)
Suzuki, H. (JP)
Katoh, A. (JP)
Ohbuchi, T. (JP)
Otsubo, H. (JP)
Dayanithi, Govindan (UEM-P) RID
Murphy, D. (JP)Source Title Endocrinology - ISSN 0013-7227
Roč. 150, č. 12 (2009), s. 5633-5638Number of pages 6 s. Language eng - English Country US - United States Keywords transcription ; protein fusion gene ; hypothalmus Subject RIV FH - Neurology CEZ AV0Z50390512 - UEM-P (2005-2011) AV0Z50390703 - UEM-P (2007-2013) UT WOS 000272042300048 DOI 10.1210/en.2009-0796 Annotation The up-regulation in the expression of mRNA or protein encoded by the c-fos gene is widely used as a marker of neuronal activation elicited by various stimuli. To facilitate the detection of activated neurons, we generated transgenic rats expressing a fusion gene consisting of c-fos coding sequences in frame with monomeric red fluorescent protein 1 (mRFP1) under the control of c-fos gene regulatory sequences (c-fos-mRFP1 rats). In these double-transgenic rats, almost all eGFP neurons in the supraoptic nucleus and PVN expressed nuclear mRFP1 fluorescence 90 min after hypertonic saline administration. Furthermore, when combined with transgenes expressing another fluorophore under the control of cell-specific regulatory sequences, activation of specific neuronal cell types in response to physiological cues can be readily detected. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2010
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