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Reactive Landing of Gas-Phase Ions as a Tool for the Fabrication of Metal Oxide Surfaces for In Situ Phosphopeptide Enrichment
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SYSNO ASEP 0340264 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Reactive Landing of Gas-Phase Ions as a Tool for the Fabrication of Metal Oxide Surfaces for In Situ Phosphopeptide Enrichment Author(s) Blacken, G. R. (US)
Volný, Michael (MBU-M) ORCID
Diener, M. (US)
Jackson, K. E. (US)
Ranjitkar, P. (US)
Maly, D. J. (US)
Tureček, F. (US)Source Title Journal of the American Society for Mass Spectrometry. - : American Chemical Society - ISSN 1044-0305
Roč. 20, č. 6 (2009), s. 915-926Number of pages 12 s. Language eng - English Country US - United States Keywords TANDEM MASS-SPECTROMETRY ; SELECTIVE DETECTION ; PHOSPHOPROTEOME ANALYSIS Subject RIV EE - Microbiology, Virology CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000266466600004 DOI 10.1016/j.jasms.2009.01.006 Annotation Zirconium, titanium, and hafnium oxide-coated stainless steel surfaces are fabricated by reactive landing of gas-phase ions produced by electrospray ionization of group IVB metal alkoxides. The Surfaces are used for in situ enrichment of phosphopeptides before analysis by matrix-assisted laser desorption ionization (MALDI) mass spectrometry. To evaluate this method we characterized ZrO2, (zirconia) Surfaces by (1) comparison with the other group IVB metal oxides of TiO2, (titania) and HfO2, (hafnia), (2) morphological characterization by SEM image analysis, and (3) dependence of phosphopeptide enrichment on the metal oxide layer thickness Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2010
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