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Identification of molecular targets for selective elimination of TRAIL-resistant leukemia cells. From spots to in vitro assays using TOP15 charts
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SYSNO ASEP 0338760 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Identification of molecular targets for selective elimination of TRAIL-resistant leukemia cells. From spots to in vitro assays using TOP15 charts Author(s) Petrák, J. (CZ)
Toman, O. (CZ)
Šimonová, T. (CZ)
Halada, Petr (MBU-M) RID, ORCID
Čmejla, R. (CZ)
Klener, P. (CZ)
Živný, J. (CZ)Source Title Proteomics. - : Wiley - ISSN 1615-9853
Roč. 9, č. 22 (2009), s. 5006-5015Number of pages 10 s. Language eng - English Country DE - Germany Keywords Biomedicine ; Drug resistance ; HL-60 Subject RIV CE - Biochemistry CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000272391300001 DOI 10.1002/pmic.200900335 Annotation The resistance of malignant cells to chemotherapy calls for the development of novel anti-cancer drugs. TNF-related apoptosis-inducing ligand (TRAIL) is a pro-apoptotic cytokine, which selectively induces apoptosis in malignant cells. We derived two TRAIL-resistant HL-60 subclones, HL-60/P1 and HL-60/P2, from a TRAIL-sensitive HL-60 acute promyelocytic leukemia cell line. To identify therapeutically exploitable weaknesses of the TRAIL-resistant leukemia cells that could be used as molecular targets for their elimination, we performed proteomic (2-DE) analysis and compared both TRAIL-resistant subclones with the original TRAIL-sensitive HL-60 cells. We identified over 40 differentially expressed proteins. To significantly narrow the lists of candidate proteins, we excluded proteins that are known to be often differentially expressed, regardless of experiment type and tissue (the so-called TOP15 proteins) Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2010
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