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Evaluation of different methods detecting intracellular generation of free radicals
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SYSNO ASEP 0338604 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Evaluation of different methods detecting intracellular generation of free radicals Title Hodnocení různých metod pro měření volných radikálů v buňce Author(s) Wilhelm, J. (CZ)
Vytášek, R. (CZ)
Ošťádalová, Ivana (FGU-C) RID
Vajner, L. (CZ)Source Title Molecular and Cellular Biochemistry. - : Springer - ISSN 0300-8177
Roč. 328, 1-2 (2009), s. 167-176Number of pages 10 s. Language eng - English Country US - United States Keywords radical oxygen species ; fluorescent probes ; autofluorescence Subject RIV ED - Physiology R&D Projects 1M0510 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA305/08/0108 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000267592600020 DOI 10.1007/s11010-009-0086-5 Annotation The fluorescent probes DHE, DCFH, DHR and the autofluorescence were used for detection of the developmental changes of ROS production in rabbit lens epithelial cells and in thin sections of rat heart. The probes revealed the age differences in ROS production. The results confirmed the applicability of the methods for detection of ROS generation Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2010
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