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Utilization of high-accuracy FTICR-MS data in protein quantitation experiments

SYSNO ASEP	0338590
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Utilization of high-accuracy FTICR-MS data in protein quantitation experiments
Title	Použití FTICR-MS dat naměřených s vysokým stupněm rozlišení při proteinové kvantifikaci
Author(s)	Strohalm, Martin (MBU-M) Novák, Petr (MBU-M)_{RID, ORCID} Pompach, Petr (MBU-M)_{RID, ORCID} Man, Petr (MBU-M)_{RID, ORCID} Kavan, Daniel (MBU-M)_{RID, ORCID} Witt, M. (DE) Džubák, P. (CZ) Hajdúch, M. (CZ) Havlíček, Vladimír (MBU-M)_{RID, ORCID}
Source Title	Journal of Mass Spectrometry. - : Wiley - ISSN 1076-5174 Roč. 44, č. 11 (2009), s. 1565-1570
Number of pages	6 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	mass spectrometry ; protein quantitation ; workflow
Subject RIV	EE - Microbiology, Virology
R&D Projects	LC07017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000272150300003
DOI	10.1002/jms.1602
Annotation	Human acute T-lymphoblastic leukemia cell line (CEM) trated with cisplatin, and the stabel isotype labeling by amino acids in cell culture (SILAC) strategy were used to present an improved method of data processing in high-accuracy mass spectrometry (MS). By using peptide mass fingerprinting with low mass tolerance, we were able to to utilize far more data retained in MA scans which would normally be missed by a standard processing method
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2010

Number of the records: 1