Number of the records: 1

Testing of the influenza virus purification by CIEF

SYSNO ASEP	0337690
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Testing of the influenza virus purification by CIEF
Author(s)	Horká, Marie (UIACH-O)_{RID, ORCID} Kubíček, O. (CZ) Kubesová, Anna (UIACH-O)_{RID, ORCID} Kubíčková, Z. (CZ) Rosenbergová, K. (CZ) Šlais, Karel (UIACH-O)_{RID, ORCID}
Number of authors	6
Source Title	Electrophoresis. - : Wiley - ISSN 0173-0835 Roč. 31, č. 2 (2010), s. 331-338
Number of pages	8 s.
Language	eng - English
Country	DE - Germany
Keywords	capillary isoelectric focusing ; PCR ; purification efficiency
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	IAAX00310701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z40310501 - UIACH-O (2005-2011)
UT WOS	000274431700008
DOI	10.1002/elps.200900310
Annotation	In this contribution Equine and Swine influenza viruses from the infected allantoic fluid from specific pathogen free embryonated chicken eggs were precipitaded by PEG 6000, sodium chloride and the impurities with different densities were removed in the sucrose gradients. The purification efficiency of this technique and the isoelectric points of tested viruses were determined by capillary isoelectric focusing, the purification efficiencies were compared with the results from PCR.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2010

Number of the records: 1