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Comparison of Various Types of Stationary Phases in Non-Aqueous Reversed-Phase High-Performance Liquid Chromatography–Mass Spectrometry of Glycerolipids in Blackcurrant Oil and Its Enzymatic Hydrolysis Mixture
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SYSNO ASEP 0337499 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparison of Various Types of Stationary Phases in Non-Aqueous Reversed-Phase High-Performance Liquid Chromatography–Mass Spectrometry of Glycerolipids in Blackcurrant Oil and Its Enzymatic Hydrolysis Mixture Title Porovnání různých typů stacionární fáze v nevodné HPLC-MS glycerolipidů v oleji černého rybízu a v enzymatickém hydrolyzátu oleje Author(s) Lísa, M. (CZ)
Holčapek, M. (CZ)
Sovová, Helena (UCHP-M) RID, ORCID, SAISource Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1216, č. 47 (2009), s. 8371-8378Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords ultra high-performance liquid chromatography ; C30 stationary phase ; triacylglycerols Subject RIV CB - Analytical Chemistry, Separation CEZ AV0Z40720504 - UCHP-M (2005-2011) UT WOS 000271838000016 DOI 10.1016/j.chroma.2009.09.060 Annotation Various stationary phases were tested for the analysis of complex mixture of triacylglycerols (TGs) in blackcurrant oil using non-aqueous reversed-phase system with acetonitrile–2-propanol mobile phase. Compared to conventional C18 column, retention times of TGs were reduced almost 10 times without the loss of the chromatographic resolution using ultra high-performance liquid chromatography. The separation on C30 column shows an unusual phenomenon, the dependence of the retention order of TGs on the column temperature. The sample throughput on the commercial monolithic column modified with C18 was increased at cost of low resolution. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2010
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