Number of the records: 1
Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity
- 1.
SYSNO ASEP 0337489 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity Author(s) Doubnerová, V. (CZ)
Müller, Karel (UEB-Q) RID, ORCID
Čeřovská, Noemi (UEB-Q) RID, ORCID
Synková, Helena (UEB-Q) RID, ORCID
Spoustová, Petra (UEB-Q)
Ryšlavá, H. (CZ)Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 10, č. 8 (2009), s. 3583-3598Number of pages 16 s. Language eng - English Country US - United States Keywords NADP-malic enzyme ; Nicotiana tabacum L. ; Potato virus Y strain NTN (PVYNTN) Subject RIV EI - Biotechnology ; Bionics R&D Projects 1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000269191800017 DOI 10.3390/ijms10083583 Annotation The effect of biotic stress induced by viral infection ( Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants ( Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVYO. The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVYNTN strain than in the case of strain PVYO. The relevance of NADP-malic enzyme in plants under stress conditions was discussed. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
Number of the records: 1