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The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves
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SYSNO ASEP 0337488 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves Title Regulace a mechanismus katalýzy NADP dependentní malátdehydrogenasy (dekarboxylační) z listů tabáku Author(s) Doubnerová, V. (CZ)
Potůčková, L. (CZ)
Müller, Karel (UEB-Q) RID, ORCID
Ryšlavá, H. (CZ)Source Title Journal of the Serbian Chemical Society - ISSN 0352-5139
Roč. 14, 8-9 (2009), s. 893-906Number of pages 14 s. Language eng - English Country CS - CS Keywords NADP-malic enzyme ; macroergic compounds ; Nicotiana tabacum L. Subject RIV ED - Physiology CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000270267200005 DOI 10.2298/JSC0909893D Annotation The non-photosynthetic NADP-malic enzyme EC 1.1.1.40 (NADP-ME), which catalyzes the oxidative decarboxylation of L-malate and NADP(+) to produce pyruvate and NADPH, respectively, and which could be involved in plant defense responses, was isolated from Nicotiana tabacum L. leaves. The mechanism of the enzyme reaction was studied by the initial rate method and was found to be an ordered sequential one. Regulation possibilities of purified cytosolic NADP-ME by cell metabolites were tested. Intermediates of the citric acid cycle (a-ketoglutarate, succinate, fumarate), metabolites of glycolysis (pyruvate, phosphoenolpyruvate, glucose-6-phosphate), compounds connected with lipogenesis (coenzyme A, acetyl-CoA, palmitoyl-CoA) and some amino acids (glutamate, glutamine, aspartate) did not significantly affect the NADP-ME activity from tobacco leaves. In contrast, macroergic compounds (GTP, ATP and ADP) were strong inhibitors of NADP-ME. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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