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Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter
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SYSNO ASEP 0337485 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter Title Stimulace kvetení a větvení v chryzantémách exprimujících gen ipt pod kontrolou 0.821 kb fragmentu promotoru genu LEACO1 Author(s) Khodakovskaya, M. (RU)
Vaňková, Radomíra (UEB-Q) RID, ORCID
Malbeck, Jiří (UEB-Q) RID, ORCID
Li, A. (US)
Li, Y. (US)
McAvoy, R. (US)Source Title Plant Cell Reports. - : Springer - ISSN 0721-7714
Roč. 28, č. 9 (2009), s. 1351-1362Number of pages 12 s. Language eng - English Country DE - Germany Keywords ipt Gene ; Cytokinins ; Transgenic chrysanthemum Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000269208100006 DOI 10.1007/s00299-009-0735-x Annotation The cytokinin biosynthesis gene, isopentenyl transferase (ipt), under the control of a 821 bp fragment of the LEACO1 gene promoter (from Lycopersicon esculentum) was introduced into Dendranthema x grandiflorium ‘Iridon’ (chrysanthemum). LEACO10.821kb-ipt transgenic lines exhibited in the vegetative state enhanced branching and reduced internode lengths. In the generative state, transformed lines exhibited elevated flower bud counts (3.8 – 6.7x more than WT), which was associated with a delay of flower bud development and a decrease in flower bud diameter. RT-PCR analysis indicated differences in ipt gene expression between individual transgenic lines, as well as at different stages of generative shoot development. Expression of ipt in transgenic lines correlated well with high concentrations of endogenous cytokinins. In general, transgenic lines accumulated more cytokinins, including cytokinin O-glucosides and N-glucosides in generative shoots than in the vegetative ones. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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