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Outcome of Treatment of Human HeLa Cervical Cancer Cells With Roscovitine Strongly Depends on the Dosage and Cell Cycle Status Prior to the Treatment
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SYSNO ASEP 0337483 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Outcome of Treatment of Human HeLa Cervical Cancer Cells With Roscovitine Strongly Depends on the Dosage and Cell Cycle Status Prior to the Treatment Author(s) Wesierska-Gadek, J. (AT)
Borza, A. (AT)
Walzi, E. (AT)
Kryštof, Vladimír (UEB-Q) RID, ORCID
Maurer, M. (AT)
Komina, O. (AT)
Wandl, S. (AT)Source Title Journal of Cellular Biochemistry. - : Wiley - ISSN 0730-2312
Roč. 106, č. 5 (2009), s. 937-955Number of pages 19 s. Language eng - English Country US - United States Keywords APOPTOSIS ; CELL CYCLE ARREST ; CYCLIN-DEPENDENT KINASES Subject RIV ED - Physiology CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000264664800022 DOI 10.1002/jcb.22074 Annotation Exposure of asynchronously growing human HeLa cervical carcinoma cells to roscovitine (ROSC), a selective cyclin-dependent kinases (CDKs) inhibitor, arrests their progression at the transition between G(2)/M and/or induces apoptosis. The outcome depends on the ROSC concentration. At higher close ROSC represses HPV-encoded E7 oncoprotein and initiates caspase-dependent apoptosis. Inhibition of the site-specific phosphorylation of survivin and Bad, occurring at high-dose ROSC treatment, precedes the onset of apoptosis and seems to be a prerequisite for cell death. Considering the fact that in HeLa cells the G(1)/S restriction checkpoint is abolished by E7, we addressed the question whether the inhibition of CDKs by pharmacological inhibitors in synchronized cells would be able to block the cell-cycle in G, phase. For this purpose, we attempted to synchronize cells by serum withdrawal or by blocking of the mitotic apparatus using nocodazole. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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