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Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development
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SYSNO ASEP 0337404 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development Title Translace regulovaná mRNA asociovanými faktory v průběhu maturace bovinních oocytů a časného embryonálního vývoje Author(s) Siemer, C. (DE)
Smiljakovic, T. (DE)
Bhojawni, M. (DE)
Kubelka, Michal (UZFG-Y) RID, ORCID
Tomek, W. (DE)Source Title Reproduction in domestic animals. - Malden : Wiley-Blackwell, 2009 / Rodriguez-Martinez Heriberto ; Iaverne Marcel - ISSN 0936-6768 Pages s. 35-36 Number of pages 2 s. Action 42nd Annual Conference of Physiology and Pathology of Reproduction/ 34th Mutual Conference on Veterinary and Human Reproductive Medicine Event date 26.02.2009-27.02.2009 VEvent location Leipzig Country DE - Germany Event type WRD Language eng - English Country US - United States Keywords Oocyte maturation ; Bovine embryo Subject RIV ED - Physiology R&D Projects GA524/07/1087 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) Annotation Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos. In conclusion, the results indicate that, at the transition from oocyte to embryonic development, translational initiation is regulated by striking differences in the abundance and/or phosphorylation of 5'-end and 3'-end mRNA associated factors, mainly the poly-(A) bindings proteins PABP1 and 3, their repressor PAIP2 and a Maskin-like protein with distinct eIF4E-binding properties which prevents eIF4E/cap binding and eIF4F formation in vitro. Nevertheless, from the M II stage to 16-cell embryos a substantial amount of eIF4E and, to a lesser extent, of eIF4G was precipitated by (7)m-GTP-Separose indicating eIF4F complex formation. Therefore, it is likely that in general the reduction in PABP1 and 3abundance represses overall translation during early embryonic development. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
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