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Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development
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SYSNO ASEP 0337303 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development Author(s) Siemer, C. (DE)
Smiljakovic, T. (DE)
Bhojawni, M. (DE)
Leiding, C. (DE)
Kanitz, W. (DE)
Kubelka, Michal (UZFG-Y) RID, ORCID
Tomek, W. (DE)Source Title Molecular Reproduction and Development - ISSN 1040-452X
Roč. 76, č. 12 (2009), s. 1208-1219Number of pages 12 s. Language eng - English Country US - United States Keywords In Vitro maturation ; Poly(A) binding protein ; Initiation-factor 4E Subject RIV ED - Physiology R&D Projects GA524/07/1087 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000271361500012 DOI 10.1002/mrd.21096 Annotation Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
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