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Phospholipase D family interactions with the cytoskeleton: isoform delta promotes plasma membrane anchoring of cortical microtubules
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SYSNO ASEP 0337286 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Phospholipase D family interactions with the cytoskeleton: isoform delta promotes plasma membrane anchoring of cortical microtubules Author(s) Andreeva, Z. (AU)
Ho, A. Y. Y. (AU)
Barthet, M. M. (AU)
Potocký, Martin (UEB-Q) RID, ORCID
Bezvoda, R. (CZ)
Žárský, Viktor (UEB-Q) RID, ORCID
Marc, J. (AU)Source Title Functional Plant Biology. - : CSIRO Publishing - ISSN 1445-4408
Roč. 36, č. 7 (2009), s. 600-612Number of pages 13 s. Language eng - English Country US - United States Keywords Allium ; Arabidopsis ; F-actin-microtubule interactions Subject RIV EB - Genetics ; Molecular Biology R&D Projects IAA601110916 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000267601900003 DOI 10.1071/FP09024 Annotation Phospholipase D (PLD) is a key enzyme in signal transduction-mediating plant responses to various environmental stresses including drought and salinity. Isotype PLD delta interacts with the microtubule cytoskeleton, although it is unclear if, or how, each of the 12 PLD isotypes in Arabidopsis may be involved mechanistically. We employed RNA interference in epidermal cells of Allium porrum L. (leek) leaves, in which the developmental reorientation of cortical microtubule arrays to a longitudinal direction is highly sensitive to experimental manipulation. Using particle bombardment and transient transformation with synthetic siRNAs targeting AtPLD alpha, beta, gamma, delta, epsilon and zeta, we examined the effect of 'cross-target' silencing orthologous A. porrum genes on microtubule reorientation dynamics during cell elongation. Co-transformation of individual siRNAs together with a GFP-MBD microtubule-reporter gene revealed that siRNAs targeting AtPLD delta promoted, whereas siRNAs targeting AtPLD. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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