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Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions

SYSNO ASEP	0337283
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions
Author(s)	Krinke, Ondřej (UEB-Q) Flemr, M. (CZ) Vergnolle, Ch. (FR) Collin, S. (FR) Renou, J. P. (FR) Taconnat, L. (FR) Yu, A. (FR) Burketová, Lenka (UEB-Q)_{RID, ORCID} Valentová, O. (CZ) Zachowski, A. (FR) Ruelland, E. (FR)
Source Title	Plant Physiology. - : Oxford University Press - ISSN 0032-0889 Roč. 150, č. 1 (2009), s. 424-436
Number of pages	13 s.
Language	eng - English
Country	US - United States
Keywords	SYSTEMIC ACQUIRED-RESISTANCE ; TRANSCRIPTION FACTOR ; INNATE IMMUNITY
Subject RIV	CE - Biochemistry
R&D Projects	GA203/05/0559 GA ČR - Czech Science Foundation (CSF)
	LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000265661700035
DOI	10.1104/pp.108.133595
Annotation	Salicylic acid (SA) plays a central role in defense against pathogen attack, as well as in germination, flowering, senescence, and the acquisition of thermotolerance. In this report we investigate the involvement of phospholipase D (PLD) in the SA signaling pathway. In presence of exogenous primary alcohols, the production of phosphatidic acid by PLD is diverted toward the formation of phosphatidylalcohols through a reaction called transphosphatidylation. By in vivo metabolic phospholipid labeling with P-33(i), PLD activity was found to be induced 45 min after addition of SA. We show that incubation of Arabidopsis (Arabidopsis thaliana) cell suspensions with primary alcohols inhibited the induction of two SA-responsive genes, PATHOGENESIS-RELATED1 and WRKY38, in a dose-dependent manner. This inhibitory effect was more pronounced when the primary alcohols were more hydrophobic. Secondary or tertiary alcohols had no inhibitory effect.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2010

Number of the records: 1