Number of the records: 1

Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction

SYSNO ASEP	0337170
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction
Title	Isolace nových konjugátů kyseliny indolyl-3-octové pomocí imunoafinitní extrakce
Author(s)	Pěnčík, Aleš (UEB-Q)_{ORCID, RID, SAI} Rolčík, Jakub (UEB-Q) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Magnus, V. (HR) Barták, P. (CZ) Buchtík, Roman (UEB-Q) Salopek-Sondi, N. (HR) Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Source Title	Talanta. - : Elsevier - ISSN 0039-9140 Roč. 80, č. 2 (2009), s. 651-655
Number of pages	5 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Immunoaffinity extraction ; High-performance liquid chromatography (HPLC) ; Tandem mass spectrometry (MS/MS)
Subject RIV	CE - Biochemistry
R&D Projects	GD522/08/H003 GA ČR - Czech Science Foundation (CSF)
	KAN200380801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000271701900037
DOI	10.1016/j.talanta.2009.07.043
Annotation	An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a highly specific immunoaffinity extraction with a sensitive and selective LC-MS/MS analysis. By using internal standards for each of the studied compounds, IAA and seven amino acid conjugates were analyzed in quantities of fresh plant material as low as 30 mg. In seeds of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmol/g fresh weight (IAA) to 0.44 pmol/g fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe and Val from higher plants is reported here for the first time.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2010

Number of the records: 1