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Micropropagation of Wild Service Tree (Sorbus torminalis [L.] Crantz): The Regulative Role of Different Aromatic Cytokinins During Organogenesis

SYSNO ASEP	0337010
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Micropropagation of Wild Service Tree (Sorbus torminalis [L.] Crantz): The Regulative Role of Different Aromatic Cytokinins During Organogenesis
Title	Mikropropagace Sorbus torminalis [L.] Crantz: regulační role různých aromatických cytokininů v průbehu organogeneze
Author(s)	Malá, J. (CZ) Máchová, P. (CZ) Cvrčková, H. (CZ) Karady, Michal (UEB-Q)_ORCID Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Mikulík, Jaromír (UEB-Q)_ORCID Hauserová, Eva (UEB-Q) Greplová, Jarmila (UEB-Q)_ORCID Strnad, Miroslav (UEB-Q)_{RID, ORCID} Doležal, Karel (UEB-Q)_{RID, ORCID}
Source Title	Journal of Plant Growth Regulation. - : Springer - ISSN 0721-7595 Roč. 28, č. 4 (2009), s. 341-348
Number of pages	8 s.
Language	eng - English
Country	US - United States
Keywords	Wild service tree ; Micropropagation ; Rooting
Subject RIV	EF - Botanics
R&D Projects	GA206/07/0570 GA ČR - Czech Science Foundation (CSF)
	1M06030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000271791200005
DOI	10.1007/s00344-009-9099-2
Annotation	The influences of three different aromatic cytokinin on in vitro multiplication and rhizogenesis of the wild service tree (Sorbus torminalis [L.] Crantz) were compared. The highest micropropagation was achieved on media containing BAP. On the other hand, the best rooting microcuttings were multiplied on a medium containing MeoBAPR. To compare these results with the levels of endogenous cytokinins, a newly developed UPLC-MS/MS method was used to determine 50 cytokinin metabolites in explants. The concentration of BAP9G, an important metabolite suspected to be responsible for inhibition of rooting and acclimatization problems, was found to be the highest in microcuttings grown on BAP. This agrees well with the results of rooting experiment. Levels of the most active endogenous isoprenoid cytokinins were also suppressed in these explants. This may be the result of a very high BAP uptake into the explants grown on this cytokinin.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2010

Number of the records: 1