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Quantification of acetaldehyde and carbon dioxide in the headspace of malignant and non-malignant lung cells in vitro by SIFT-MS
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SYSNO ASEP 0336742 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Quantification of acetaldehyde and carbon dioxide in the headspace of malignant and non-malignant lung cells in vitro by SIFT-MS Title Kvantifikace acetaldehydu a oxidu uhličitého ve výparech malignantních a nemalignantních plicních buněk in vitro pomocí SIFT-MS Author(s) Sule-Suso, J. (GB)
Pysanenko, Andriy (UFCH-W) RID, ORCID
Španěl, Patrik (UFCH-W) RID, ORCID
Smith, D. (GB)Source Title Analyst. - : Royal Society of Chemistry - ISSN 0003-2654
Roč. 134, č. 12 (2009), s. 2419-2425Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords acetaldehyde ; SIFT-MS ; carbon dioxide Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GA202/09/0800 GA ČR - Czech Science Foundation (CSF) GA203/09/0256 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000271864900007 DOI 10.1039/b916158a Annotation Previous studies using selected ion flow tube mass spectrometry, SIFT-MS, showed that CALU-1 lung cancer cell cultures emit acetaldehyde in proportion to the number of cells in the culture medium. However, studies in another laboratory led to conflicting results, so these SIFT-MS studies have been repeated and extended to include NL20 normal lung epithelial cells and 35FL121 Tel+ telomerase positive lung fibroblast cells. Thus, SIFT-MS has been used to quantify acetaldehyde and, additionally, carbon dioxide, acetone and ethanol in the headspace of the cell culture medium alone and the headspace of the appropriate medium containing 50 x 10(6) or 80 x 10(6) cells following incubation for 16 h at 37 degrees C. Three independent experiments were carried out for each cell type for both cell numbers and for each of the two culture media used. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2010
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