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Gene Specific Molecular Markers for Hop (Humulus lupulus L.)
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SYSNO ASEP 0336095 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Gene Specific Molecular Markers for Hop (Humulus lupulus L.) Author(s) Patzak, J. (CZ)
Matoušek, Jaroslav (BC-A) RID, ORCIDNumber of authors 2 Source Title Proceedings of the Second International Humulus Symposium. - Ghent : ISHA Acta Horticulturae, 2009 / DeKeukeleire D. ; Hummer K.E. ; Heyerick A. - ISSN 0567-7572 - ISBN 978-90-6605-722-7 Pages s. 73-80 Number of pages 8 s. Action ISHS International Humulus Symposium /2./ Event date 01.09.2009-05.09.2009 VEvent location Ghent Country BE - Belgium Event type WRD Language eng - English Country BE - Belgium Keywords cluster analysis ; expressed sequence tag microsatellite repeat (EST-SSR) ; single nucleotide polymorphism (SNP) Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA521/08/0740 GA ČR - Czech Science Foundation (CSF) QH81052 GA MZe - Ministry of Agriculture (MZe) CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) Annotation New efficient and reliable expressed sequence tag microsatellite repeat (EST-SSR) molecular markers have been identified. Forty primer pairs for 29 gene-specific sequences were evaluated for identification and clustering in eight hops cultivars. Gene-specific markers based clustering was compared to clustering based on SSR and AFLP markers and on a combination of molecular markers. Gene-specific markers were showed to be ideal for DNA fingerprinting, for control of authenticity and purity of varieties, for evaluation of genetic diversity in genetic resources, etc. They are readily applicable in breeding, genetic linkage mapping and quantitative trait loci (QTL) analysis. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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