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Polyamine metabolism during the cell cycle of synchronized tobacco BY-2 cell line
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SYSNO ASEP 0335900 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Polyamine metabolism during the cell cycle of synchronized tobacco BY-2 cell line Title Metabolismus polyaminů v průběhu buněčného cyklu synchronní buněčné kultury tabáku BY-2 Author(s) Gemperlová, Lenka (UEB-Q) ORCID, RID
Cvikrová, Milena (UEB-Q) RID, ORCID
Fischerová, Lucie (UEB-Q) RID, ORCID
Binarová, Pavla (MBU-M) RID, ORCID
Fischer, L. (CZ)
Eder, Josef (UEB-Q)Source Title Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428
Roč. 47, č. 7 (2009), s. 584-591Number of pages 8 s. Language eng - English Country FR - France Keywords ADC ; Cell cycle ; DAO Subject RIV EF - Botanics R&D Projects IAA500200719 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50380511 - UEB-Q (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000267194800005 DOI 10.1016/j.plaphy.2009.02.014 Annotation The time courses of the contents of free, soluble and insoluble polyamine (PA) conjugates, PA biosynthetic and catabolic enzyme activities and mRNA levels of PA biosynthetic genes were monitored during the cell cycle of synchronized tobacco BY-2 cell line (Nicotiana tabacum L. cv. Bright Yellow 2). Progression through the cell cycle was characterized by specific biphasic changes of PA levels. The first, moderate peak in the amount of free PAS coincided with the S-phase. After a transient decline in G2 phase the contents of free PAS increased rapidly and peaked again during G2/M interface. Then sharply decreased with the minimum at the end of mitosis and during M/G1 transition and started to rise again with the next replication phase. Levels of PA soluble conjugates paralleled those of the free forms. Biosynthetic enzyme activities followed the biphasic manner analogous to the levels of free PAS and seemed to be regulated on both transcriptional and (post)translational level. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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