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The N-terminal region is crucial for the thermostability of the G-domain of Bacillus stearothermophilus EF-Tu
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SYSNO ASEP 0335710 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The N-terminal region is crucial for the thermostability of the G-domain of Bacillus stearothermophilus EF-Tu Author(s) Šanderová, Hana (UMG-J)
Tišerová, Hana (UMG-J)
Barvík, I. (CZ)
Sojka, Luděk (UMG-J)
Jonák, Jiří (UMG-J)
Krásný, Libor (UMG-J)Number of authors 6 Source Title Biochimica Et Biophysica Acta-Proteins and Proteomics. - : Elsevier - ISSN 1570-9639
Roč. 1804, č. 1 (2010), s. 147-155Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords thermostability ; G protein ; EF-Tu ; G-domain ; Bacillus Subject RIV EB - Genetics ; Molecular Biology R&D Projects KJB500520601 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) NR9138 GA MZd - Ministry of Health (MZ) 2B06065 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000272765200020 DOI 10.1016/j.bbapap.2009.09.024 Annotation Bacterial elongation factor Tu (EF-Tu) is a model monomeric G protein composed of 3 covalently linked domains. Previously, we evaluated the contributions of individual domains to the thermostability of EF-Tu from thermophilic bacterium Bacillus stearothermophilus. We showed that domain 1 (G-domain) sets up the basal level of thermostability for the whole protein. Here we located the thermostability determinants distinguishing the thermophilic domain 1 from mesophilic domain 1. By systematically swapping protein regions differing between G-domains from mesophilic B. subtilis and thermophilic B. stearothermophilus we demonstrate that a small portion of the protein, the N-terminal 12 aa residues, plays a key role in the thermostability of this domain. The thermostabilizing effect of the N-terminal region could be mediated by stabilizing the functionally important effector region and the effect of the N-terminal region is significant also for the thermostability of full-length EF-Tu. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2010
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